| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality
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| Autores: |
Fleury, Elodie
Moal, Jeanne
Boulo, Viviane
Daniel, Jean-yves
Mazurais, David
Henaut, Alain
Corporeau, Charlotte
Boudry, Pierre
Favrel, Pascal
Huvet, Arnaud
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| Data: |
2010-06
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| Ano: |
2010
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| Palavras-chave: |
Mollusca
Bivalvia
Gene expression
CDNA microarrays
Reproduction
Oxidative stress
Energy metabolism
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| Resumo: |
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00005/11590/8258.pdf
DOI:10.1007/s10126-009-9227-9
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| Editor: |
Springer
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| Relação: |
http://archimer.ifremer.fr/doc/00005/11590/
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| Formato: |
application/pdf
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| Fonte: |
Marine Biotechnology (1436-2228) (Springer), 2010-06 , Vol. 12 , N. 3 , P. 326-339
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| Direitos: |
2010 Springer. Part of Springer Science+Business Media
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